N John Sushma,
Sri Padmavati Women’s University, India
Title: Rivastigmine loaded solid lipid nanoparticles: Formulation and in vitro characterization and antioxidant activity
Biography
Biography: N John Sushma,
Abstract
Injectable biodegradable nanoparticles have an important potential application in the treatment of a variety of neurological disorders. Rivastigmine is an oral medication used to treat patients with Alzheimer’s disease. It is a short acting cholinesterase inhibitor (ChEI) and blocks the action of acetylcholinesterase, the enzyme responsible for the destruction of acetylcholine. The purpose of the present study was to formulate and evaluate rivastigmine loaded solid lipid nanoparticles for sustained release. In the present study, rivastigmine loaded solid lipid nanoparticles were prepared using fish oil and flax seed oil by melting emulsification coupled with high shear homogenization technique. Variable drug/polymer ratios in nine different batches were taken to identify the suitable lipid ratios for better entrapment efficiency of the drug and also for sustained release of the drug. The prepared nanoparticles were evaluated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS) and atomic force microscopy (AFM). The particle size of the SLNs was found to exponentially decrease with the increase in surfactant solution. Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) and differential Scanning calorimetry (DSC) analysis revealed that there was no chemical interaction between the ingredients of SLNs. Further drug entrapment efficiency and in vitro release studies were carried out. Scanning electron microscopy results revealed that the nanoparticles were spherical in shape. In vitro release studies showed that rivastigmine loaded SLNs were capable of releasing the drug in a sustained manner. In vitro antioxidant activity of SLNs and different concentrations of rivastigmine alone was determined by reducing power assay and DPPH activity. The experimental results showed the suitability of SLNs as a potential carrier for providing sustained delivery of rivastigmine.
