Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 15th World Medical Nanotechnology Congress Osaka, Japan.

Day 1 :

Keynote Forum

Stoyan Sarg Sargoytchev

World Institute for Scientific Exploration, USA

Keynote: Atlas of atomic nuclear structures according to the BSM-super-gravitation unified theory

Time : 09:10-09:50

Medical Nanotechnology 2017 International Conference Keynote Speaker Stoyan Sarg Sargoytchev photo
Biography:

Stoyan Sarg Sargoytchev has completed his Master’s degree in Electrical Engineering from Technical University, Sofia and PhD in Physics from Bulgarian Academy of Sciences. He has worked with Canadian government institutions and universities and retired from York University. Currently, he is with the World Institute for Scientific Exploration. He has published more than 70 scientific papers in reputed journals and he is an author of a theoretical monograph BSM-SG.

 

Abstract:

The atlas of atomic nuclear structures (ANS) derived by the basic structures of matter-super-gravitation unified theory (BSM-SG), illustrates the super-dense structure of the elementary particles and atomic nuclei. Their real size and configuration appears hidden due to the revealed space micro-curvature around the atomic nuclei. While they exhibit the same interaction energies as the quantum mechanical models, they are not point-like structures. The atlas provides information about the spatial arrangement of the protons and neutrons in the atomic nuclei. The Z-number trend of the nuclear build-up follows a shell structure that complies strictly with the row-column pattern of the periodic table. They possess identifiable features of oxidation numbers and obey the Hund's rules and Pauli Exclusion Principle. The trend of fast increase in the number of neutrons in comparison to the protons in heavier elements and their spatial positions plays a role in redistribution of the repulsive Coulomb forces between protons. The nuclear structures of the stable isotopes exhibit a higher degree of symmetry with a classical explanation of the nuclear spin. This gives a reasoning why some isotopes are stable and others are not. The proposed physical models could be useful for deeper understanding of the nuclear transmutations and they could be applied in chemistry, biomolecules and different fields of nanotechnology.

Keynote Forum

Shaker A Mousa

Albany College of Pharmacy and Health Sciences, USA

Keynote: Impact of nano-biotechnology on the future of medicine (nanomedicine): The road toward precision medicine

Time : 09:50-10:30

Medical Nanotechnology 2017 International Conference Keynote Speaker Shaker A Mousa photo
Biography:

Shaker A Mousa finished his PhD from Ohio State University, College of Medicine, Columbus, OH and Post-doctoral Fellowship, University of Kentucky, Lexington KY. He also received his MBA from Widener University, Chester, PA. He is currently an endowed tenure Professor and Executive Vice President and Chairman of the Pharmaceutical Research Institute and Vice Provost for Research at ACPHS. Prior to his academic career, he was a senior Scientist and fellow at The DuPont Pharmaceutical Company for 17 years, where he contributed to the discovery and development of several FDA approved and globally marketed diagnostics and therapeutics. He holds over 350 US and International Patents discovering novel anti-angiogenesis strategies, antithrombotics, anti-integrins, anti-cancer, and non-invasive diagnostic imaging approaches employing various nanotechnology platforms. His has published more than 1,000 journal articles, book chapters, published patents, and books as editor and author. He is a member of several NIH study sections, and the Editorial Board Member of several high impact Journals. His research has focused on diagnostics and therapeutics of angiogenesis-related disorders, thrombosis, vascular and cardiovascular diseases.

Abstract:

Over the past few years, evidence from the scientific and medical communities has demonstrated that nanotechnology and nanomedicine have tremendous potential to profoundly impact numerous aspects of cancer and other disorders in term of early diagnosis and targeted therapy. The utilization of nanotechnology for the development of new nano-carrier systems has the potential to offer improved chemotherapeutic delivery through increased solubility and sustained retention. One of the major advantages of this cutting-edge technology is its unique multifunctional characteristics. Targeted delivery of drug incorporated nanoparticles, through conjugation of tumor-specific cell surface markers, such as tumor-specific antibodies or ligands, which can enhance the efficacy of the anticancer drug and reduce the side effects. Additionally, multifunctional characteristics of the nano-carrier system would allow for simultaneous imaging of tumor mass, targeted drug delivery and monitoring (Theranostics). A summary of recent progress in nanotechnology as it relates specifically to nanoparticles and anti-cancer drug delivery will be reviewed. Nano nutraceuticals using combination of various natural products provide a great potential in diseases prevention. Additionally, various nanomedicine approaches for the detection and treatment of various types of organ specific delivery, vascular targeting and vaccine will be briefly discussed.

Keynote Forum

Hussein O Ammar

Future University, Egypt

Keynote: New trends in nanotechnology-based targeted drug delivery systems

Time : 10:30-11:10

Medical Nanotechnology 2017 International Conference Keynote Speaker Hussein O Ammar photo
Biography:

Holder of the First Class Golden Medal for Sciences and Arts and the recipient of the 2010Appreciation State Prize in the realm of Advanced Technological Sciences.Professor Ammar is currently the Chairman, Pharmaceutical Technology Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt; formerly, Dean of the Pharmacy Division, National Research Centre, Cairo, Egypt. He has more than 110 research papers published in international scientific journals. These research papers cover most of the areas related to pharmaceutics, biopharmaceutics and pharmacokinetics. Design of new drug delivery systems is not beyond the scope of his interest.

Abstract:

In recent years, theranostics are emerging as the next generation of multifunctional nanomedicine to improve the therapeutic outcome of cancer therapy. Polymeric nanoparticles with targeting moieties containing magnetic nanoparticles as theranostic agents have considerable potential for the treatment of cancer.  The use of directed enzyme prodrug therapy (DEPT) has been investigated as a means to improve the tumor selectivity of therapeutics. Magnetic DEPT involves coupling the bioactive prodrug-activating enzyme to magnetic nanoparticles that are then selectively delivered to the tumor by applying an external magnetic field.  Gene therapy is an attractive method for meeting the needs for curing brain disorders, such as Alzheimers disease and Parkinsons disease. On the other hand, due to the fact that hepatocellular carcinoma (HCC) is resistant to standard chemotherapeutic agents, gene therapy appears to be a more effective cure for HCC patients. Ultrasound-mediated drug delivery is a novel technique for enhancing the penetration of drugs into diseased tissue beds non-invasively. This technique is broadly appealing, given the potential of ultrasound to control drug delivery spatially and temporally in a non-invasive manner.

Keynote Forum

Amit Banerjee

Shizuoka University, Japan

Keynote: Room-Temperature Terahertz Microbolometer Arrays for Biomedical Imaging Applications

Time : 11:30-12:10

Medical Nanotechnology 2017 International Conference Keynote Speaker  Amit Banerjee  photo
Biography:

 

Dr. Amit Banerjee has received Ph.D. degree in Semiconductor Technology from Energy Research Unit, Indian Association for the Cultivation of Science (D.S.T., Govt. of India). Currently He is working in the Advanced Device Research Division, Research Institute of Electronics, Shizuoka University, National University Corporation, Japan as a Scientific Researcher. His current research interest is on Terahertz Technology, including THz devices (sensors and sources) fabrication, materials engineering, and optimization for surveillance, inspection, biomedical, imaging and deep space exploration applications.  Amit has co-authored several scientific papers, presented in several international conferences among lead speakers, received several awards including Young Physicist Award, Award by the Metrology Society of India (MSI), Indian Institute of Chemical Engineers (IIChE), Award by Dept. of Atomic Energy (D.A.E.), his work was also featured as a Key Scientific Article contributing to excellence in engineering, scientific and industrial research. Amit is member JSAP, IPS, along with other many International Advisory Committees, Technical Program Committees, acted as Panel Editor, Reviewer for reputed journals.

Abstract:

Terahertz waves lie in the region from 300 GHz to 3 THz (wavelength: 100 µm to 1 mm). Electromagnetic wave with frequency range ~ 1 THz, because of its transparency to many non-polarized materials and finger-print spectral bands of organic- and biomacromolecules, is important for non-contact and non-destructive sensing in various applications. Diverse technologies are using these frequency bands for ultrahigh-speed wireless communications, imaging, and materials analysis. There are several exciting applications of THz spectroscopy and imaging e.g. non-contact and remote inspection of concealed weapons, explosives for homeland security, examination of defects and foreign objects in edibles. Another exciting aspect is the development of THz imaging devices, techniques and instruments for biomedical applications for disease diagnostics at a level of cellular processes and tissues, including cancer signature study, diagnosis of tumors, by thermography and imaging. Other various biomedical applications of THz range from studying biomolecules, including analysis of DNA/RNA, amino acids/peptides, proteins, and carbohydrates etc. However, the present ability of THz technology is still inadequate for actual use in large scale. The overall performance in terms of sensitivity and speed of measurements is unsatisfactory and the manufacturing cost of even the basic devices is not commercially viable. Farther research and development of the sources, detectors, optical elements and measuring techniques as well as sensing systems are crucial for extending the utilization of THz waves. Among lot of issues, the current study details THz detectors and detection systems with promising results in terms of performance such as sensitivity and response speed, convenience in handling and prospective low-cost development. Microbolometer is a radiation detector for infrared (IR) and terahertz (THz) waves. The current report is on the detailed investigation of the materials properties, design requirement and device performance aspects, for the fabrication of uncooled antenna-coupled terahertz microbolometer arrays to be used for biomedical imaging applications.

  • Nanomedicine and Drug Delivery | Drug Delivery and Therapeutics | Biosensors, Diagnostics and Imaging | Advanced Nanomaterials | DNA Nanotechnology
Speaker

Chair

Hussein Ammar

Future University, Egypt

Speaker

Co-Chair

Anthony William

University of Tokyo, Japan

Session Introduction

Anthony William

University of Tokyo, Japan

Title: Observing the molecular mechanical properties of DNA

Time : 12:10-12:30

Speaker
Biography:

Anthony William Coleman has completed his BA in Chemistry and  DPhil from the University of Sussex. He has authored 300 research articles, over 120 invited talks and 25 patents. He has numerous international projects underway in particular with the University of Tokyo elected FRSC in 2010.

Abstract:

The mechanical properties of DNA control both its containment in the nucleus and also the processes of transcription and construction of proteins. Hence, it is clear that knowledge of the properties that is a key to understanding how life is built. However, how to carry out such measurements without perturbing the inherent structure and properties of DNA is a major problem. Classical and even modern methods require modifying the chemical nature of DNA and hence its properties. The discovery of how microfluidics can be applied to silicon nano tweezer (SNT) measurement of the mechanical properties of non-modified DNA is a major step forward. Here, we will present how SNT technology can be used to study events changing the mechanical properties of DNA-(1) How physiological cations change selectively the mechanics of DNA as a function of the physiological localization, (2) The effects of DNA coordinating systems on DNA mechanics and how ion/bioactive couples can have amplified action and (3) The action of DNA coordinating nucleosomes on the structure of DNA.

 

 

Jinn P Chu

National Taiwan University of Science and Technology, Taiwan

Title: Thin film metallic glass: A promising coating for biomedical applications

Time : 12:30-12:50

Speaker
Biography:

Jinn P Chu is a Professor of Materials Science and Engineering, National Taiwan University of Science and Technology (NTUST), Taiwan. He has completed his PhD in Materials Science from University of Illinois at Urbana-Champaign and has received the Excellence in Research Award in 2008 and became a Distinguished Professor at NTUST in 2012.

Abstract:

A new group of thin film metallic glasses (TFMGs) have been reported to show properties different from conventional crystalline metal films, though their bulk forms are already well-known for high strength and toughness, large elastic limits, excellent wear and corrosion resistance owing to their amorphous structure. In addition, the smooth surface, due to the grain boundary-free structure and low surface free energy of TFMGs can be achieved and lead to the relatively high hydrophobicity and the low coefficient of friction. In our studies, TFMG coatings are deposited using RF magnetron sputtering for various biomedical applications, including the property enhancements of dermatome blades and syringe needles as well as the antibacterial property. The TFMG-coated dermatome blades show great enhancements in sharpness and durability, compared with those of the bare one. For the syringe needle, the insertion and retraction forces were measured when needles were inserted into phantom materials, including polyurethane (PU) rubber block and pork muscle with a constant needle speed. TFMG-coated needle showed a significant reduction in both forces of ~66% and ~72%, respectively, which were significantly lower than those of bare needle during testing against the PU rubber. Furthermore, the bacterial adhesion of Escherichia coli and Staphylococcus aureus to both Zr- and Cu-based TFMGs is hindered to different extents. Good performances of TFMG and the antibacterial property are thought to be caused by the relatively low surface free energy and low coefficient of friction. Thus, TFMG coating appears to be a promising candidate for biomedical applications.

 

Miriam Colombo

University of Milano-Bicocca, Italy

Title: Oral nanocarrier for insulin colon delivery

Time : 13:50-14:10

Speaker
Biography:

Miriam Colombo obtained her PhD in Biology in 2012 at the university of Milano-Bicocca. From September 2013 she is Assistant Professor in Clinical Biochemistry at the Dep. Biotechnology and Bioscience of University of Milano-Bicocca, Italy.She is author of 44 peer-reviewed scientific publications.

Abstract:

The current treatment of diabetes disease relies on insulin subcutaneous injection . Because of parenteral administration drawbacks, alternative administration routes have been investigated . Among all, the oral administration may lead to a better glucose regulation exploiting the liver first-pass metabolism of insulin, thus preventing the risks of fluctuating glycaemia. However, the oral bioavailability of peptides is very low and several efforts have been attempted to promote insulin bowel absorption. Despite all, the oral delivery of insulin remains an unmet need .The aim of work was to prepare, characterize and evaluate both in vitro and in vivo a novel nanoformulated multiple-unit colon delivery system, i.e. coated pellets, as a possible oral nanocarrier for insulin. Insulin-loaded polymeric nanoparticles (NPs) were synthesized according to previously published protocols with some improvements . The driving force of NPs formation was the opposite charges of polyethyleneimine and dextran sulphate resulting in the insulin entrapment into the polymeric matrix. NPs were incorporated into cores that were subsequently coated with three overlapping layers, aiming to release insulin into the large intestine: this gastrointestinal site is indeed characterized by a relatively low proteolytic activity. The system was evaluated in vitro for its physico-technological characteristics, NPs dispersion, disintegration and release performance, showing delayed release behavior. Finally, the coated nanoformulation effect was tested in diabetic rats: a significant hypoglycaemic activity, due to the synergistic effect of NPs and colon delivery, was observed. In this study, a new approach for the oral administration of insulin is proposed. The synergistic effect due to the nanoformulation of insulin and the encapsulation in a triple-layer pellet system for colon-release delivery results in a significant and long-lasting hypoglycemic effect. The impact of our multitasking macromolecule delivery system for oral insulin in controlling diabetes is clinically appealing, since it represents an oral route for insulin administration, with a prolonged hypoglycemic activity and a more physiological insulin metabolism.

Hoonsung Cho

Chonnam National University, South Korea

Title: Protamine conjugated fluorochromes: A new photosensitizer for photodynamic tumor therapy

Time : 14:10-14:30

Speaker
Biography:

Hoonsung Cho is an Assistant Professor at Chonnam National University. He has earned his PhD degree from University of Cincinnati in 2010. He has joined Center for Advanced Medical Imaging Sciences at Massachusetts General Hospital in Boston, Massachusetts and extended his research into the field of multifunctional nanocarrier systems for imaging, targeting and therapy. His current research interests include imaging cell death with multimodal vital fluorochromes and detecting extracellular DNA and RNA using fluorochrome-functionalized nanoparticles as probes for detection and manipulation of these nucleic acids.

 

 

Abstract:

The Photodynamic therapy (PDT) is a promising alternative therapy that could be used adjunct to chemotherapy and surgery for curing cancer causing tissue destruction by visible light in the presence of a photosensitizer (PS) and oxygen. The high arginine peptides like the cell-penetrating peptide have membrane translocating and nuclear localizing activities that have led to their use in a wide range of drug delivery applications. Protamine is a high arginine peptide with membrane translocating and nuclear localizing properties. The reaction of an NHS-ester of methylene Blue (MB) and clinical protamine (Pro), to yield MB-Pro, was described in this context and demonstration of phototoxicity which clinical protamine improved PDT effect was performed. The reaction between clinical protamine (Pro) an NHS ester of MB is a solution phase reaction with the complete modification of the protamine peptides which feature a single reactive amine at the N-terminal proline and single carboxyl group at the C-terminal arginine. The aim of this study was to find a new type of photosensitizer (PS) for PDT on in vitro and in vivo experiments and to assess the anti-tumor effect of PDT using the protamine conjugated-PS on the cancer cell line. Photodynamic cell death studies show that the MB-Pro produced has more efficient photodynamic activities than MB alone, causing rapid light induced cell death. The attachment of MB to clinical Pro, yielding MB-Pro, confers the membrane internalizing activity of its high arginine content on methylene blue and can induce a rapid photodynamic cell death, presumably due to cell membrane rupture induced by light. The PDT using MB-Pro for HT-29 cells was very effective and those findings suggest that MB-Pro is one of candidate for photosensitizer in solid tumors.

Speaker
Biography:

Dr. Jessica A. Bertolini is a PhD in the Prof. Davide Prosperi’s NanoBioLab at the University of Milano-Bicocca. Her researcher activity is focused on biomedical applications of Nanotechnology, specifically on the use of cellular and molecular approaches to optimize and test nanoparticles, developed to be carrier of therapeutic agents, in cell cultures. Her current scientific interests concern the use of molecular biology techniques to prepare and modify DNA plasmids associated to nanoparticles, and cell biology techniques to test nanoparticles in cell cultures.

Abstract:

The improvements in nanomedicine are strictly dependent on the development of methods to deliver therapeutic agents specifically and efficiently to the target cells with minimal toxicity. One of the main mechanisms by which the nanoparticles are able to enter into mammalian cells takes advantage of an endocytic pathway of internalization. Nowadays, one of the principal problems that occur to the use of nanoparticles in nanomedicine is the degradation of the nanoparticles-associated therapeutic agents into cellular lysosomes, due to the difficulty for them to escape the endosomal pathway. Thus, the study of the endosomal escape mechanism becomes crucial to the development of efficient drug delivery nanosystems for therapeutic treatments .We developed a new approach to measure and quantify the endosomal escape mechanism of nanoparticles, using poly(isobutylene-alt-maleic anhydride)-graft-dodecyl amine (PMA) polymer normally used in our laboratory for colloidal nanoparticle coating. This synthetic polymer proved to be able to trigger the nanoparticle endosomal escape in previous investigations. A biarsenical fluorescent label fluorophore, called CrAsH, is linked to the PMA nanoparticles. The CrAsH fluorophore is non-fluorescent as such but, upon binding with proteins containing a tetra-cysteine motif Cys-Cys-Pro-Gly-Cys-Cys, becomes fluorescent (2;3;4). Cell cultures are previously transfected with a plasmid that encodes for a Blue Fluorescent Protein (5) linked to the tetra-cysteine motif in C-terminal region and later treated with PMA+CrAsH nanoparticles. The double fluorescence detection provides the quantification of the nanoparticles fraction, linked to the CrAsH fluorophore, able to reach the cytosol and recognize the tetra-cysteine target motif. Hence, this gives us a real measurement and quantification of the nanoparticle ratio able to really trigger the endosomal escape providing, thus, an estimation of the amount of therapeutic agents, potentially linked to the nanoparticles, that are actually able to reach the cellular cytoplasm and be available for medical treatment.

Speaker
Biography:

Dr. Yuqi Yang received her PhD in 2015 from Central China Normal University, and then joined Wuhan Institute of Physics and Mathematics as an assistant proferssor. Dr. Yang’s study focuses on multi-modality contrast agents, especially specializes in nanomaterial-based agents for molecular imaging of tagrted cancer cells.

 

Abstract:

As a noninvasive molecular imaging modality, optical imaging provides high sensitivity and specificity but poor tissue penetration depth. On the contrary, MRI has high spatial resolution in deep tissue, but it suffers the drawbacks of low sensitivity. The combination of MRI and optical imaging, which takes advantages of both modalities, has received widespread attention in biological and medical application. Compared to conventional 1H-MRI, 19F-MRI displays an intense sensitivity (0.83 relative to 1H) and negligible background signal. Herein, we report a one-pot microwave synthesis of a functionalized 19F CA, fluorinated silicon nanoparticles (19F-SiNP), for targeted detection of A549 lung cancer cells. Moreover, based on the quantum effects of the nano-sized nanoparticles, the 19FSiNP can also act as a label free dye for ultracontrast fluorescent imaging. In vitro and in vivo results shows that the peptide-conjugated 19FSiNP can specifically detect A549 cells for dual 19F-MRI and fluorescence imaging. Such functionalized 19FSiNP can also be labeled to other cancer cells by replacing the targeting group, providing a handy and reliable way for targeted dual-model imaging.

 

Elham Ahmadi

Tabriz University of Medical Sciences, Iran

Title: PLGA-PAA copolymers: Their size and structure influenced drug delivery applications

Time : 15:50-16:10

Speaker
Biography:

Elham Ahmadi is currently pursuing her Masters in Medical Biotechnology at Tabriz University of Medical Sciences and Bachelor’s degree in Cellular and Molecular Biology from Azarbijan Shahid Madani University, Tabriz. She is currently working on a project of nanotechnology based drug delivery for anti-cancer targeting and treatment.

 

Abstract:

Statement of the Problem: The need for high cellular drug uptake and reduce the adverse effects of chemotherapeutic drugs obligate us to design and prepare a suitable nano-carrier. Poly (lactide-co-glycolic acid) PLGA is one of the most well used polymer for the development of biocompatible nanoparticles.Purpose: The purpose of the study is to prepare the PLGA-PAA (Poly-lactide-co-glycolide)- (poly acrylic acid) co-polymer which is the first new developed biocompatible nano-carrier until now. We co-encapsulated hydrophilic doxorubicin and hydrophilic hydroxytyrosol in PLGA-PAA biocompatible nano-capsules.Methodology & Theoretical Orientation: We synthesized the poly (acrylic acid) (PAA) polymer by radical polymerization method in the presence of dry tetrahydrofuran as solvent of the reaction. Then the PLGA-PAA copolymer was synthesized by ring opening polymerization method in the presence of stannous Octanoate (snoct2) as catalyst of the reaction. An oil-in-water emulsion process was utilized for the encapsulation of hydrophilic doxorubicin and hydroxytyrosol.Findings: FTIR confirmed the PAA and PLGA-PAA copolymer formation is correct. HNMR, CNMR also approved it. The size of PLGA-PAA nanoparticles is measured 24 nm by DLS technique and a spherical particle with an average diameter of 16 nm is determined by SEM technique. The amount of drug loading and unloading is computed.Conclusion & Significance: PLGA-PAA co-polymer was found to be a promising co-polymer for the preparation of nanoparticles with small size distribution and relatively high drug loading. Formulation of functionalized PLGA-PAA nanoparticles leads to enhance the high drug loading efficiency of hydrophilic drugs.

Fatemeh sultani

Mashhad University of Medical Sciences, Iran

Title: In vitro evaluation of peptide-cholesterol hybrid as gene delivery nano-carrier

Time : 15:50-16:10

Speaker
Biography:

Fatemeh Soltani has completed her PhD in Pharmaceutical Biotechnology from Mashhad University of Medical Sciences, Iran. She has worked on the synthesis of lipid-peptide core nanoparticles by using solid phase peptide synthesis (SPPS) methods. Currently, she is an Assistant Professor in Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Iran. Her research focuses on discovering efficient peptide, polymer or lipid-based drug/gene delivery vectors which are produced using either biological or synthetic methods.

 

Abstract:

Gene therapy has the potential to compensate the abnormal genes and treat both genetic and acquired disorders. For a successful gene therapy, a gene of interest must enter the cells successfully. To achieve this, an efficient and safe carrier is needed. Viral vectors are naturally based carriers and most widely used systems. Although they are efficient in gene transferring but they suffer from immunogenicity and toxicity. On the other hand, the non-viral counterparts such as lipids, proteins, polymers and peptides are safe enough to be considered as gene carriers for clinical application. However, their efficiency should be improved. Cationic peptides in particular to those derived from nature are important because they are biodegradable and biocompatible. They can be designed in order to develop multifunctional carriers having various functional motifs to improve gene delivery efficiency. Nuclear proteins such as protamine and histone are able to condense DNA and have been adopted in several gene delivery systems. However, the major concern related to these vectors is the risk of immunogenicity. To solve this drawback, some small peptides preserving the gene delivery properties of the whole protein have been chosen. In this study, we designed a cationic vector composed of a small cationic peptide derived from protamine as condensing agent and histidine tag for escaping from endosome. In order to form a micelle like particle, a cholesterol molecule was introduced to the N- terminal of the vector. The synthesized vector was evaluated in terms of structure, gene delivery efficiency and toxicity by using various standard methods. Based on the results the vector could form nano-micelle, condense pGFP and deliver it to the nucleus successfully. In addition, it showed negligible toxicity in comparison to PEI-25 kDa. Overall, further in vivo investigations are needed to shed light on the usage of these carriers in clinic.

Speaker
Biography:

Ramin Banan Sadeghian is an Assistant Research Professor in WPI-AIMR, Tohoku University. His expertise background and training are in microelectronics, VLSI processes, micro- and nano-fabrication, gas sensors, biosensors, semiconductor electron devices, thermo-electrics, instrumentation and industrial automation.

 

 

Abstract:

Superoxide anions as a member of reactive oxygen species (ROS) are involved in various physiological and pathological states. For instance, the rate of superoxide generation from skeletal muscle tissue is known to increase with contractile activity, fatigue and aging. It is therefore, very important to selectively detect and accurately quantify the release rates of super-oxides within both physiological and pathological levels. I will report on fabrication and characterization of enzyme-functionalized electrochemical superoxide biosensors built on a thick film of nanoporous gold and a three-dimensional macro-porous mesh of nanoporous gold. The devices were first tested and calibrated offline and then employed to detect superoxide release rates from C2C12 myoblast cells and myotubes upon stimulation with an endogenous superoxide producing drug. Two to three orders of magnitude higher sensitivities were achieved as compared to those of earlier reported devices.

 

Speaker
Biography:

Dr. Shizhen Chen received her PhD in 2011 from Central China Normal University, and then began her post-doctoral research at Wuhan Institute of Physics and Mathematics. In 2013, she became an associate professor. Dr. Chen’s work focuses on magnetic resonance imaging (MRI) contrast agents, especially concentrated on the nanomaterial-based MRI contrast agents for lung cancer imaging.  

 

Abstract:

The rational design of theranostic nanocomposite exhibiting synergistic turn-on of therapeutic potency and enhanced diagnostic imaging in response to tumor milieu is critical for efficient personalized cancer therapy.We here in fabricate a theranostic nanocomposites Mn-porphyrin & Fe3O4@SiO2@PAA-cRGD, which could synergistically enhancing positive and negative magnetic resonance imaging signals for both accurate tumor imaging and pH-responsive drug release. Fluorescent imaging also showed that the nanocomposite specifically accumulated in lung cancer cells by a receptor-mediated process, and were nontoxic to normal cells. The r2/r1 ratio varied is 20.6 in neutral pH 7.4 , r2/r1 ratio 7.7 in acidic pH 5.0, which suggesting the NCs could act as an ideal T1/T2 dual-mode contrast agents at acidic environments of tumor areas. For in vivo MRI, T1 and T2 relaxation was significantly accelerated to 55% and 37%, respectively, in the tumor after i.v. injection of nanocomposites. The results demonstrate great potential of such nanocomposites for real-time imaging with greatly enhanced diagnostic accuracy during targeted therapy.